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多功能DNA修复酶重组兔单克隆抗体
产物名称 | 英文名称 | 货号 |
多功能DNA修复酶重组兔单克隆抗体 | APEX1 | GOY-01K0102 |
商品属性:
英文名称: APEX1
中文名:多功能DNA修复酶重组兔单克隆抗体
别 名;APEX1_HUMAN;
DNA-(apurinic or apyrimidinic site) endonuclease; EC:3.1.11.2; APE; APE1; APEX;
APX; HAP1; REF1; APEX nuclease (APEN); Apurinic-apyrimidinic endonuclease 1 (AP
endonuclease 1; APE-1); REF-1; Redox factor-1; DNA-(apurinic or apyrimidinic
site) endonuclease, mitochondrial;
研究领域;心血管 染色质和核信号 转录调节因子 表观遗传学
抗体来源;Rabbit
克隆类型;Monoclonal
克 隆 号R2D12
交叉反应;(predicted:
Human, Mouse, Rat, )
产物应用;WB=1:1000-5000
IHC-P=1:100-500 IHC-F=1:200-500 (石蜡切片需做抗原修复)
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理论分子量;36kDa
细胞定位;细胞核 细胞浆
性 状;Liquid
浓 度;1mg/ml
免 疫 原;KLH
conjugated synthetic peptide derived from human APEX1
亚 型;IgG
纯化方法;affinity
purified by Protein A
保存条件;Shipped at
4℃. Store at -20 °C for one
year. Avoid repeated freeze/thaw cycles.
注意事项;This
product as supplied is intended for research use only, not for use in human,
therapeutic or diagnostic applications.
产物介绍:
The APEX gene encodes the major AP endonuclease in
human cells. It encodes the APEX endonuclease, a DNA repair enzyme with apurinic/apyrimidinic
(AP) activity. Such AP activity sites occur frequently in DNA molecules by
spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that
remove specific abnormal bases. The AP sites are the most frequent
pre-mutagenic lesions that can prevent normal DNA replication. Splice
variants have been found for this gene; all encode the same protein.
Disruptions in the biological functions related to APEX are associated with
many various malignancies and neurodegenerative diseases.[provided by RefSeq,
Dec 2019] Subcellular Location: Nucleus. Nucleus, nucleolus. Nucleus speckle.
Endoplasmic reticulum. Cytoplasm. Note=Detected in the cytoplasm of B-cells
stimulated to switch. Colocalized with SIRT1 in the nucleus. Colocalized with
YBX1 in nuclear speckles after genotoxic stress. Together with OGG1 is
recruited to nuclear speckles in UVA-irradiated cells. Colocalized with
nucleolin and NPM1 in the nucleolus. Its nucleolar localization is cell cycle
dependent and requires active rRNA transcription. Colocalized with
calreticulin in the endoplasmic reticulum. Translocation from the nucleus to
the cytoplasm is stimulated in presence of nitric oxide (NO) and function in
a CRM1-dependent manner, possibly as a consequence of demasking a nuclear export
signal (amino acid position 64-80). S-nitrosylation at Cys-93 and Cys-310
regulates its nuclear-cytosolic shuttling. Ubiquitinated form is localized
predominantly in the cytoplasm. DNA-(apurinic or apyrimidinic site) lyase,
mitochondrial: Mitochondrion. Note=The cleaved APEX2 is only detected in
mitochondria. Translocation from the cytoplasm to the mitochondria is
mediated by ROS signaling and cleavage mediated by granzyme A.
Tom20-dependent translocated mitochondrial APEX1 level is significantly increased
after genotoxic stress. |
多克隆抗体制备:
我公司合成抗原,保证阳性血清和抗体贰尝滨厂础效价达到1:32000,由蛋白抗原制备的多抗,保证奥叠检测结果是阳性。技术服务实行分步报价,按节点收费,为客户节约成本。
鼠单克隆抗体制备:
客户可根据项目实际需求情况灵活选择进行,我公司将为您的项目提供专业的建议。在制备过程中,与客户互动验证,确保最终制备的抗体贰尝滨厂础阳性血清检测达到合同要求的同时,也能满足客户抗体最终应用验证需求。技术服务实行分步报价,按节点收费,为客户节约成本。
重组抗体表达服务:
重组抗体技术使制备人源化抗体和人源抗体成为可能,而这是其他常规的多克隆或单克隆抗体制备方法无法做到的。我公司已搭建好的抗体测序、重组抗体表达等先进平台,能为客户提供各种类型的重组抗体表达服务,包含鼠源单克隆抗体构建及表达、更换恒定区亚型的嵌合抗体表达,抗体人源化改造及表达等服务。
单项免疫学及检测服务:
我公司也为客户提供单项免疫学和检测服务,如动物免疫、动物饲养、抗体修饰服务(如贵滨罢颁,贬搁笔等的标记)、以及贰尝滨厂础,奥叠,滨贬颁,顿叠等检测服务。
抗体定制服务:
已注册商标,主要用于抗体药物发现和开发,是我公司针对有特定应用要求和各种模型测试的客户量身定制的专业服务,主要面向工业客户。
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