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血蓝蛋白抗体
产物名称 | 英文名称 | 货号 |
血蓝蛋白抗体 | KLH | GOY-01K0105 |
商品属性：
英文名称: KLH
中文名:血蓝蛋白抗体
别 名；Keyhole limpet
hemocyanin (KLH); KLH 1 ; KLH1; Keyhole limpet hemocyanin A; KLH-A; Hemocyanin
1; hemocyanin A chain.
研究领域；免疫学
抗体来源；Mouse
克隆类型；Polyclonal
产物应用；WB=1:500-2000
ELISA=1:5000-10000
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理论分子量；800-900kDa
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH
protein: full length
亚 型；IgG
纯化方法；affinity
purified by Protein A
缓 冲 液；0.01M
TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件；Shipped at
4℃. Store at -20 °C for one
year. Avoid repeated freeze/thaw cycles.
产物介绍:
Keyhole limpet hemocyanin is an extremely large,
heterogeneous glycosylated protein consisting of subunits with a molecular
weight of 350,000 and 390,000 in aggregates with molecular weights of
4,500,000-13,000,000. Each domain of a KLH subunit contains two copper atoms
that together bind a single oxygen molecule (O2). When oxygen is bound to
hemocyanin, the molecule takes on a distinctive transparent, opalescent blue
color. The KLH protein is potently immunogenic yet safe in humans and is therefore
highly prized as a vaccine carrier protein. The large and highly glycosylated
KLH protein cannot be reproduced synthetically. It is available only as a
purified biological product from the Keyhole Limpet Megathura crenulata. Keyhole limpet hemocyanin (KLH) is used
extensively as a carrier protein in the production of antibodies for
research, biotechnology and therapeutic applications. Haptens are substances
with a low molecular weight such as peptides, small proteins and drug
molecules that are generally not immunogenic and require the aid of a carrier
protein to stimulate a response from the immune system in the form of
antibody production.[2] KLH is the most widely employed carrier proteins for
this purpose. KLH is an effective carrier protein for several reasons. Its
large size and numerous epitopes generate a substantial immune response, and
abundance of lysine residues for coupling haptens, allows a high
hapten:carrier protein ratio increasing the likelihood of generating
hapten-specific antibodies. In addition, because KLH is derived from the
limpet, a gastropod, it is phylogenetically distant from mammalian proteins,
thus reducing false positives in immunologically based research techniques in
mammalian model organisms. KLH may also be a challenging molecule to work
with because of its propensity to aggregate and precipitate. Aggregates
remain immunogenic, but limit the ability to conjugate haptens and are
difficult to manipulate in the laboratory. A high quality KLH preparation
with the clear opalescent blue color is the best indicator of KLH solubility. |
多克隆抗体制备：
我公司合成抗原，保证阳性血清和抗体贰尝滨厂础效价达到1：32000，由蛋白抗原制备的多抗，保证奥叠检测结果是阳性。技术服务实行分步报价，按节点收费，为客户节约成本。
鼠单克隆抗体制备:
客户可根据项目实际需求情况灵活选择进行，我公司将为您的项目提供专业的建议。在制备过程中，与客户互动验证，确保最终制备的抗体贰尝滨厂础阳性血清检测达到合同要求的同时，也能满足客户抗体最终应用验证需求。技术服务实行分步报价，按节点收费，为客户节约成本。
重组抗体表达服务:
重组抗体技术使制备人源化抗体和人源抗体成为可能，而这是其他常规的多克隆或单克隆抗体制备方法无法做到的。我公司已搭建好的抗体测序、重组抗体表达等先进平台，能为客户提供各种类型的重组抗体表达服务，包含鼠源单克隆抗体构建及表达、更换恒定区亚型的嵌合抗体表达，抗体人源化改造及表达等服务。
单项免疫学及检测服务:
我公司也为客户提供单项免疫学和检测服务，如动物免疫、动物饲养、抗体修饰服务（如贵滨罢颁，贬搁笔等的标记）、以及贰尝滨厂础，奥叠，滨贬颁，顿叠等检测服务。
抗体定制服务:
已注册商标，主要用于抗体药物发现和开发，是我公司针对有特定应用要求和各种模型测试的客户量身定制的专业服务，主要面向工业客户。
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